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. 2016 Feb 22;7(13):16180–16193. doi: 10.18632/oncotarget.7568

Figure 4. YAP signaling is required for LATS1-induced inhibition of cell growth and invasion.

Figure 4

(A) YAP-overexpressed vector was transfected into LATS1-pretreated GC cell lines (SGC-7901 and HGC-27). After transfection for 72 h, cell proliferative activity was assessed by MTT assay, indicating that YAP overexpression weakened the therapeutic effects of LATS1 on cell growth. (B, C) After YAP-overexpressed vector was transfected into LATS1-treated GC cell lines (SGC-7901 and HGC-27), cell invasive potential was determined by Transwell assay, demonstrating that YAP lowered the anti-invasion effect of LATS1 in GC cells. (D) Western blotting analysis of the YAP, p-YAP, CTGF, MMP-9, Bax and CyclinD1 expression in YAP-transfected SGC-7901 and HGC-27 cells treated with LATS1. (E) Immunofluorescence analysis of the location of YAP in SGC-7901 and HGC-27 cells transfected with LATS1. The data were shown as the means ± SD of three independent experiments. **P < 0.01.