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. 2016 Feb 18;7(13):16194–16204. doi: 10.18632/oncotarget.7477

Figure 1. TXL solution regulates VEGF-A, ANGPT-1 and ANGPT-2 expression in TNF-α-stimulated RAW 264.7 cells.

Figure 1

A. qRT-PCR analysis of mRNA level of VEGF-A in TNF-α-stimulated RAW 264.7 cells pretreated with or without TXL solution (100, 200, 500 μg/ml). B. Representative western blot image and quantification of protein level of VEGF-A in TNF-α-stimulated RAW 264.7 cells pretreated with or without TXL solution. C. qRT-PCR analysis of relative mRNA level of ANGPT-1 in TNF-α-stimulated RAW 264.7 cells pretreated with or without TXL solution. D. Representative western blot image and quantification of protein level of ANGPT-1 in TNF-α-stimulated RAW 264.7 cells pretreated with or without TXL solution. E. qRT-PCR analysis of relative mRNA level of ANGPT-2 in TNF-α-stimulated RAW 264.7 cells pretreated with or without TXL solution. F. Representative western blot image and quantification of protein level of ANGPT-2 in TNF-α-stimulated RAW 264.7 cells pretreated with or without TXL solution. G. Quantification of the protein ratio of ANGPT-1/ANGPT-2 in TNF-α-stimulated RAW 264.7 cells pretreated with or without TXL solution. Bar 1: TNF-α only; bar 2: TNF-α+TXL 100 μg/ml; bar 3: TNF-α+TXL 200 μg/ml; bar 4: TNF-α+TXL 500 μg/ml; Data are mean ±SEM. * P<0.05 vs TNF-α only; # P<0.05 vs TNF-α+TXL 100 μg/ml.