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. 2016 Feb 24;7(13):16311–16324. doi: 10.18632/oncotarget.7684

Figure 2. Low level of DIM enhances the stemness of gastric cancer cells.

Figure 2

A. Real-time RT-PCR for the expression of Oct4, SALL4, and Sox2 genes in HGC-27 cells treated with 0, 1, and 10 μM DIM for 48 h. (n = 3, P < 0.05). B. Western blotting assays for the expression of CD44, SALL4, c-MYC, Oct4, Nanog, and Sox2 proteins in HGC-27 cells treated with 0, 1, and 10 μM DIM for 48 h. C. Immunofluorescent staining of CD44 and Sox2 proteins in HGC-27 cells treated with 0, 1, and 10 μM DIM for 48 h. Original magnification, 200Χ. D. Adipogenic differentiation of HGC-27 cells treated with 0, 1, and 10 μM DIM for 48 h. Original magnification, 200 Χ.