Table 3. Primer sequences of CREB and SKA2 for qRT-PCR and ChIP assays.
Gene | Primer sequences |
---|---|
CREB | F: 5′-GAAGATTCACAGGAGTCAGTGGATA-3′ R: 5′-GAAGATTCACAGGAGTCAGTGGATA-3′ |
gCREB | F: 5′-GTCATGGCCTACGAGGAGAA-3′ R: 5′-CACGTCAGGGAGAAGCAGAG-3′ |
SKA2 | FI: 5′-CTGAAACTATGCTAAGTGGGGGAG -3′ R1: 5′-TTCCAAACATCCTGACACTCAAAAG-3′ |
SKA2 (for CHIP) | F2: 5′-GTACACACGCATGCACACAC-3′ R2: 5′-AGGCAAGGGAAATAGGGAAA-3′ |
GAPAD | F: 5′-AAGCCTGCCGGTGACTAAC-3′ R: 5′-GCATCACCCGGAGGAGAAAT-3′ |
gGAPAD | F: 5′-GGTCATCCATGACAACTTTGG-3′ R: 5′-GGCCATCACGCCACAG-3′ |