Skip to main content
. 2016 Feb 24;7(13):16396–16408. doi: 10.18632/oncotarget.7651

Figure 5. Effect of anti-miR-199a and -214 on the heterospheroid formation and hPSC-induced tumor cell proliferation.

Figure 5

(A) Heterospheroids were prepared by co-culturing Panc-1 tumor cells and hPSCs (control or transfected with anti-miRs) together using hanging drop method. In total, 15 drops were analyzed per condition, and the experiments were performed in three independent settings. (B) Panc-1 tumor cell growth curves after incubation with conditioned medium obtained from Panc-1 (self-control), control hPSCs, and TGFβ-activated hPSCs. (C) Panc-1 tumor growth curves after incubation with conditioned medium obtained from TGFβ-activated hPSCs transfected with anti-miR-199a (50 nM) significantly reduced the Panc-1 proliferation compared to negative control anti-miR. Cell growth was measured with AlamarBlue dye. A.u. denote arbitrary unit. Data are representative of three independent experiments. Data is represented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.