Table 3. Primary and secondary antibodies used for the immune-blotting analysis.
Antibody | Source | Dilution |
---|---|---|
Mouse monoclonal α-SMA | Sigma | 1:500 |
Goat polyclonal Collagen1α1 | Southern Biotech | 1:250 |
Mouse monoclonal β-actin | Sigma | 1:10000 |
HRP-conjugated rabbit anti-goat IgG | DAKO | 1:2000 |
HRP-conjugated goat anti-mouse IgG | DAKO | 1:2000 |