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. 2016 Feb 19;7(13):16445–16461. doi: 10.18632/oncotarget.7501

Figure 1. Construction and production of mAb04-MICA fusion protein.

Figure 1

A. Structure diagram of the fusion protein. B. Flow diagram for the construction of expression plasmids. C, D. Western blot analysis for the assembling of purified mAb04-MICA. C i: anti-human IgG (H+L) antibody under nonreducing condition; C i: anti-human IgG (H+L) antibody under reducing condition. D i: anti-human MICA antibody under nonreducing condition; D i: anti-human MICA antibody under reducing condition. Lane 1: mAb04-MICA; Lane 2: mAb04; Lane 3: hMICA. E. SDS-PAGE analysis for the purity of mAb04-MICA. Ei: nonreducing condition; Ei: reducing condition. Lane 1: mAb04-MICA; Lane 2: mAb04; Lane 3: hMICA.