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. 2016 Feb 19;7(13):16445–16461. doi: 10.18632/oncotarget.7501

Figure 5. mAb04-MICA induced the G0/1 phase arrest in MDA-MB-231 cells, inhibited VEGFR2 pathway and modulated the Bcl-2 family protein.

Figure 5

A. Cell cycle analysis of MDA-MB-231 cells that were incubated with various treatments for 48 h and stained with PI. The percentage of cells in each phase was indicated. B. Quantitative analysis of cell cycle assay. C, D. Western blot analysis for p-VEGFR2/VEGFR2, p-AKT/AKT, p-ERK/ERK and p-P38/P38 of MDA-MB-231 cells treated with mAb04-MICA. Equal loading of protein was confirmed by stripping the immunoblot and reprobing it for β-actin. E. Gray scanning and data statistics of C, D. F. Western blot analysis for Bcl-2 family protein of MDA-MB-231 cells treated with mAb04-MICA. G. Gray scanning and data statistics of (F) Data were presented as the mean ± SD, n = 3 (B, E, G), *p < 0.05, **p < 0.01.