Figure 4. Stem cell-like cancer cells have sphere-forming ability.

(A) Sphere-forming ability of CD133/CD44⁻ and CD133⁺/CD44⁺ cells (upper panels). Sphere size of CD133−/CD44⁻ and CD133⁺/CD44⁺ cells (bottom panels). (B) Immunostaining of stemness markers c-Myc and Sox2 in HT29 sphere cells. (C) Sequential sphere-forming ability and continuous stemness maker expression (Scale bar = 100 μm; upper panels). Relative expression of the stemness markers Nanog (1), Sox2 (2), Oct4 (3), and c-Myc (4) in CD133⁻/CD44⁻ and CD133⁺/CD44⁺ cells (bottom panel).