Figure 5. Enhanced suppression of stem cell-like HT29 human colon cancer cells by CVV.

(A, B) Tumor sizes measured in xenograft models of 10⁶ live monolayer-cultured HT29 cells (mono HT29), sphere-cultured HT29 cells (sphere HT29), sphere HT29 cells treated with 5-Fu, and HT29 cells treated with CVV showed that CVV can attenuate tumor growth and successfully kill stem cell-like cells (n = 6–8; *p < 0.05 vs. mono HT29, t test; scale bar = 1 cm). (C) The CD133⁺/CD44⁺ double-positive SCCs population (left panel) and real-time PCR results (right panel) show that CVV can highly suppress stem cell-like HT29 cells compared to 5-Fu treatment (n = 3, *p < 0.05).