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. 2016 Feb 24;7(13):16542–16566. doi: 10.18632/oncotarget.7665

Figure 2. PE5 and PE21, but not PE4, PE6, PE8 or PE12, extend the CLS of yeast grown under CR conditions.

Figure 2

WT cells were grown in the synthetic minimal YNB medium initially containing 0.5% glucose (CR conditions) or 2% glucose (non-CR conditions), in the presence of a PE or in its absence. Survival curves (A), the mean (B) and maximum (C) lifespans of chronologically aging WT strain cultured under CR or non-CR conditions in the absence of a PE are shown; data are presented as means ± SEM (n = 5–7). CR caused significant extension of CLS (A) (p < 0.05; the p values for comparing survival curves were calculated with the help of the GraphPad Prism statistics software). CR extended both the mean (B) and maximum (C) lifespans (*p < 0.05; the p values for comparing the means of two groups were calculated with the help of the GraphPad Prism statistics software using an unpaired two-tailed t test). Survival curves of chronologically aging WT strain cultured under CR on 0.5% glucose with or without 0.5% PE4 (D), 0.5% PE5 (E), 1% PE6 (F), 0.3% PE8 (G), 0.1% PE12 (H) or 0.1% PE21 (I) are shown; data are presented as means ± SEM (n = 5–7). CLS extension under CR on 0.5% glucose was significant for PE5 and PE21 (p < 0.05; the p values for comparing survival curves were calculated with the help of the GraphPad Prism statistics software). CLS extension under CR on 0.5% glucose was not significant for PE4, PE6, PE8 and PE12. Abbreviations: Logarithmic (L), post-diauxic (PD) or stationary (ST) growth phase.