Skip to main content
. 2016 Feb 24;7(13):16542–16566. doi: 10.18632/oncotarget.7665

Figure 7. In yeast grown under non-CR conditions, PE4, PE5, PE6, PE8, PE12 and PE21 alter the patterns of age-related changes in intracellular reactive oxygen species (ROS) known to be generated mainly as by-products of mitochondrial respiration.

Figure 7

WT cells were grown in the synthetic minimal YNB medium initially containing 2% glucose, in the presence of a PE or in its absence. The intracellular concentrations of ROS were measured in live yeast by fluorescence microscopy of dihydrorhodamine 123 staining, as described in Materials and methods. Age-dependent changes in ROS concentrations within chronologically aging WT cells cultured under non-CR conditions on 2% glucose with or without 0.5% PE4 (A), 0.5% PE5 (B), 1% PE6 (C), 0.3% PE8 (D), 0.1% PE12 (E) or 0.1% PE21 (F) are shown; data are presented as means ± SEM (n = 3–4; *p < 0.05; the p values for comparing the means of two groups were calculated with the help of the GraphPad Prism statistics software using an unpaired two-tailed t test). Abbreviations: Logarithmic (L), post-diauxic (PD) or stationary (ST) growth phase.