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. 2016 Feb 22;7(13):16593–16609. doi: 10.18632/oncotarget.7565

Figure 5. B19 is found to activate the ER stress via promoting the accumulation of ROS.

Figure 5

A. Effect of B19 on the morphology of endoplasmic reticulum in SGC-7901 cells. SGC-7901 cells were treated as described in Experimental Section. The morphology of endoplasmic reticulum in SGC-7901 cells was examined with an electron microscope (×10000 or ×20000). Results from a representative cell sample out of three studied in each group are shown. The ER morphologies are normal in the control group, the B19 (20 μM)+ NAC (5 mM) group and NAC (5 mM) group (arrows indicate normal ER). Exposure to 20 μM B19 for 12 h induced mitochondrial dysfunction in B19 (20 μM) group (arrows indicate swollen ER). B. SGC-7901 cells were treated with B19 (20 μM) for the indicated times, the protein levels of ATF4, p-EIF2α and CHOP were determined by western blot. GAPDH and eIF2α were used as internal control. C. Western blot results from (B) were calculated and compared with control. D. SGC-7901 cells and BGC-823 cells were pre-incubated with or without 5 mM NAC for 2 h before exposure to B19 at the indicated concentrations, 3 hours later the ATF4, p-EIF2α, and XBP-1 expression were detected by western blot. The protein level of CHOP was examined by western blot after treatment with B19, B19+NAC pretreated, or NAC alone for 12 h. GAPDH and eIF2α were used as internal control.