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. 2016 Feb 25;7(13):16688–16702. doi: 10.18632/oncotarget.7696

Figure 3. APE1 decreases acidic bile salts-induced double strand DNA damage in EAC cells.

Figure 3

Cells were non-treated or treated with acidic bile salts (200 μM, pH 4.0) for 30 min followed by recovery in complete media for 3 h and subjected to immunofluorescence and comet assays. Immunofluorescence for p-H2AX (S139) in control adenovirus or APE1 adenovirus infected OE33 cells (A) and in control shRNA or APE1 shRNA stably transfected FLO-1 cells (B). Quantification analyses of immunofluorescence data are shown on the right panels A and B. Comet assay in OE33 cells stably expressing empty vector (pcDNA) or APE-1 (C) and FLO-1 cells stably expressing control shRNA or APE-1 shRNA (D). Quantification analyses are shown on the right panels C and D. The results are representative of at least three independent experiments.