Figure 7. Inhibition of APE1 DNA repair activity enhances DNA damage, apoptosis and activation of JNK and p38 pathways in response to acidic bile salts.

FLO-1 cells were non-treated or treated with acidic bile salts (200 μM, pH 4.0) alone or in combination with CRT (100 μM) for 30 min, followed by recovery in complete media for 3 h and subjected to AP sites and CellTiter-Glo assays, Annexin V/PI staining, and Western blotting. AP sites (A), CellTiter-Glo (B), Annexin V/PI (C), and Western blot analysis (D and E) data are shown. β-actin was used as a loading control. Quantification analysis of Annexin V/PI staining data is shown on the right panel C.