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. 2016 Feb 26;7(13):16731–16744. doi: 10.18632/oncotarget.7744

Figure 3. Treatment with ABT-737 before X-ray exposure triggers radiation-induced intrinsic apoptosis in SQ20B cell line and intra-mitochondrial oxidative stress.

Figure 3

SQ20B cells were treated with 0.1% DMSO (Control: Ctr) or 10 μM ABT-737, 20 h before a 10 Gy irradiation. After 24 h, 48 h, 72 h and 120 h, (A) cells were fixed and the percentage of TUNEL-positive cells were measured by flow cytometry analysis or (B) the percentage of cells having a caspase activity was measured on alive cells by flow cytometry analysis. (C) A Western blot analysis was performed to determine the specific activation of the procaspases-3 by cleavage. (D) The mitochondrial ROS production was validated with a positive (Antimycin A treated cells) control by fluorescence microscopy. Scale bar, 5 μm. (E) Specific mitochondrial ROS production was investigated by flow cytometry analysis using the MitoSOX labeling. (F) The loss of mitochondrial outer membrane potential (ΔΨm) was measured through a JC-1 staining on living cells. *p < 0.05; **p < 0.01; ***p < 0,001.