Skip to main content
. 2016 Feb 27;7(13):16745–16759. doi: 10.18632/oncotarget.7778

Figure 3. NOR1 suppresses the slug-induced EMT process and invasiveness of NPC cells.

Figure 3

(A) qRT-PCR analysis of the EMT transcription factor Slug and the epithelial marker keratin in NPC 5–8F, and HNE1 cells expressing control vectors pIRES or pIRES/NOR1. (B) Tumor cell migration assay. Ectopic expression of NOR1 suppresses migration of NPC 5-8F and HNE1 cells, but migration was rescued by concomitant transfection with a Slug expression plasmid. The graph represents summarized data of five independent experiments. Expression of exogenous Slug protein in transfected cells was determined by western blotting. (C) Tumor cell invasion assay. The same cells described in (B) were subjected to a tumor cell invasion assay. The graph provides summarized data of five independent experiments. **P < 0.01, ***P < 0.001 relative to the control cell data.