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. 2016 Feb 23;7(13):16818–16839. doi: 10.18632/oncotarget.7605

Figure 4.

Figure 4

a. SDS-PAGE analysis of solubility of human TCTP-intein protein (47 kDa) from ERTXB101 after expression with 0.1 mM IPTG and various temperatures (Samples were adjusted to the number of bacterial cells (3.84 × 109 cfu). Arrows indicate the expressed human TCTP-intein (47 kDa). M: Protein ladder, (Thermo Scientific), Lane 1: 37°C, 3 h, soluble proteins, Lane 2: 37°C, 3 h, insoluble proteins, Lane 3: 27°C, 5 h, soluble proteins, Lane 4: 27°C, 5 h, insoluble proteins, Lane 5: 18°C, 16 h, soluble proteins, Lane 6: 18°C, 16 h, insoluble proteins, Lane 7: 18°C, 16 h, non-induced soluble proteins, Lane 8: 18°C, 16 h, non-induced insoluble proteins. b. SDS-PAGE analysis of affinity purification of ERTXB101 by chitin column after induction of protein expression at 27°C for 5 h with washing buffers in the presence of 1 mM EDTA, M: Protein ladder (Thermo Scientific), Lane 9: loading sample, Lane 10: flow through from chitin column, Lane 11: wash, Lane 12: DTT flush to distribute it evenly throughout the column, Lane 13: elution of human TCTP (19 kDa) after stopping column flow and inducing cleavage reactions.