Table 4. Plasmid used/constructed in this study.
Plasmids | Characteristics | Sources |
---|---|---|
pTXB1 | ApR, contains a mini-intein from the Mycobacterium xenopigyrA gene (Mxe GyrA intein; 198 amino acid residues) that has been modified to undergo thiol-induced cleavage at its N-terminus. | NEB |
pTCTP01 | ApR, pTXB1 containing human seTCTP gene under the control of the T7 promoter and encoding human TCTP-intein fusion protein with an C-terminal Intein-tag. | This study |