Figure 6. Administration of exogenous TSLP inhibits tumor growth in a xenograft mouse model of human colon cancer.

SW1116 and TSLPR^kd-SW1116 cells were subcutaneously injected, respectively, into nude mice at day 0. A. Experimental procedure used to administer TSLP to nude mice bearing colon tumors. PBS-0.1%BSA was used as control. B. Tumor-bearing mice and tumors excised from each group at day 21. C. Tumor volume and tumor weight at day 21 were measured. **p<0.01 n=7. Data are presented as mean±SEM. D and E. Tumor sections from each group were stained with H&E to show necrotic area (D) and with TUNEL kit to show the apoptotic cells (E). F. Western blotting analysis of protein levels of total and cleaved caspase-3 in tumor tissue lysates.