Figure 3. Rock1 deficiency impacts integrin-mediated adhesion and migration.
(A) BMMC's from WT or Rock1−/− mice (1 × 105) were subjected to an in vitro adhesion assay on retronectin as described in methods. Adhesion was assessed by measuring absorbance at 600 nM at indicated times. Shown is pooled data from 4–6 independent experiments performed in triplicates ± SEM. *P < 0.05 for WT vs Rock1−/−. (B) BMMC's from WT or Rock1−/− mice (2.5 × 105) were subjected to an in vitro migration assay on retronectin as described in methods. Cell migration was performed either in the presence or absence of SCF (50 ng/mL) and is expressed as the average number of cells migrated ± SD. Ten fields were scored from one representative experiment. Similar results were observed in 4 independent experiments. *P < 0.05, WT vs Rock1−/−.