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. 2016 Mar 2;7(13):16936–16947. doi: 10.18632/oncotarget.7851

Figure 5. Rock1 regulates KIT mediated signaling in BMMCs.

Figure 5

Reduced activation of ROCK, LIMK, AKT and ERK MAP kinase in Rock1-deficient BMMCs. WT and Rock1−/− BMMCs were starved overnight in serum- and cytokine-free media and stimulated with SCF (100 ng/mL) for 5 min. Equal amount of protein lysates were subjected to western blot analysis using an anti-Rock1, anti-Rock2, anti-phospho-MYPT1, anti-β-actin, anti-phospho-AKT, anti-phospho-ERK1/2, anti-total AKT or anti-total ERK1/2 antibodies as indicated. Similar findings were observed in 3–4 independent experiments.