Figure 6. Effect of cisplatin treatment on viability and projected area of ovarian cancer spheroids following cisplatin treatment.

A, B. OVCAR8 spheroids generated on hanging drop arrays were significantly more chemoresistant to cisplatin (26% viable in 50 cells/drop; 59% viable in 500 cells/drop) compared to spheroids generated on ultra-low attachment plates with or without nutation (8-14% viable in 50 cells/drop; 12-30% viable in 500 cells/drop). Viability within spheroids was measured using alamarblue fluorescence and normalized to untreated control spheroids generated on the same platform. C, D. Similarly, reduction in projected area and size of cisplatin-treated spheroids was most dramatic in spheroids generated on ultra-low attachment plates, owing to higher sensitivity to cisplatin.