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. 2016 Mar 5;7(13):17009–17020. doi: 10.18632/oncotarget.7927

Figure 5. Oroxylin A induces cytosolic activation of p53 and recql4 mitochondrial translocation in HCT-116 cells.

Figure 5

(A) HCT-116 cells were incubated with the indicated concentrations of DMSO or oroxylin A (100 μM). Confocal images of the cells show the fluorescence of p53 in blue, Mito in green, Recql4 in red, and the merge in Column 4. (B) Antibodies to p53, Recql4, and Bcl-xL were used for the immunoprecipitation. Western blotting was used to detect complex formation between proteins. (C) HCT-116 cells were incubated with the indicated concentrations of DMSO, TPA (20 ng), and oroxylin A (100 μM). Confocal images of the cells show the fluorescence of p53 in blue, Bcl-xL in green, and the merged images in Column 3. (D) After the mitochondria were isolated from HCT-116 cells, Western blotting was performed to analyze Recql4, p53, and Bcl-2.