Figure 1. Quantification of VEGF, VEGFR2 and CRMP4 proteins and mRNA levels in gastric cancer tissues.
A. Western blot analysis showing increased expression levels of CRMP4, VEGF and VEGF2. B. Relative expression of CRMP4 and VEGF by western blot analysis. C. qRT-PCR to detect the relative mRNA expression levels of CRMP4, VEGF and VEGF2 in gastric cancer and tumor-adjacent tissues. All experiments were performed in triplicate. The results from 3 pairs of specimens analyzed by ANOVA are expressed as means ± SD. *P < 0.05 vs tumor-adjacent tissues. GAPDH was used as the control.