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. 2016 Feb 25;7(13):17074–17086. doi: 10.18632/oncotarget.7717

Figure 5. VEGF upregulates CRMP4 expression.

Figure 5

A. CRMP4 expression induced by an increasing concentration of VEGF. SGC7901 and HGC27 gastric cancer cells were treated with different concentrations (0 – 100 ng/mL) of VEGF for 24 hours. B. CRMP4 expression induced by increasing incubation times with a constant VEGF concentration of 20 ng/mL. SGC7901 and HGC27 gastric cancer cells were treated with 20 ng/mL of VEGF for various incubation periods ranging from 0 to 72 hours. The CRMP4 protein level was determined by Western blot analysis, and GAPDH served as an internal control. Whole-cell lysates were prepared and immunoblotted using antibodies against CRMP4.