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. 2016 Mar 6;7(13):17144–17161. doi: 10.18632/oncotarget.7942

Figure 6. Effect of oral cancer cells on focal adhesion formation in the treated siMMP-13.

Figure 6

OC3 and OC3-I5 cells were transfected with siMMP-13 and recovered after 24 h. The cells were seeded on a 12-mm glass slide and incubated overnight. Subsequently, the cells were fixed and hybridized with phalloidin for 1 h and DAPI for 1 min. (A) Photographs of the actin dots. (B) Microscopic images showing the count and quantification of the actin dots. The mock was considered the negative control. Scale bar: 20 μm. Error bars denote the mean ± SEM (n = 3). *P < .05 and ***P < .001.