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. 2016 Feb 26;7(13):17162–17181. doi: 10.18632/oncotarget.7751

Figure 2. ER maleate inhibited cell invasion and migration potential, and modulated the expression of TIMP-MMPs in OSCC cells.

Figure 2

A. ER maleate significantly inhibited invasive capability of SCC4 cells in a dose dependent manner (0 – 2 μM) after 24 h incubation by transwell invasion assay. Bar graphs show the decrease in invaded cell number with ER maleate treatment in a dose dependent manner. B. ER maleate significantly suppressed cell migration to the wound area in SCC4 cells in comparison with vehicle control cells in 24 h by wound healing assays. Histogram analysis showing significantly low number of cells in wound of ER maleate treated cells. C. ER maleate treatment decreased the expression of MMP-1, MMP-10, MMP-12 and MMP-13, while TIMP-2 expression increased with no significant change in TIMP-1 at the mRNA level in SCC4 cells analyzed by illumine mRNA profiles. The bar graph data presented as mean ± SEM; groups denoted by different letters represent a significant difference at p < 0.05(ANOVA followed by Fisher's LSD test).