Figure 3. Five human thyroid cancer cell lines were treated for 24 hrs with A.

IFNγ. alone B. PLX4720 or PD03250901 alone or in combination with IFNγ C. Quantitative PCR fold change of PD-L1 mRNA was normalized to control (A, B) or to IFNγ treatment state (C). D. Western blot analysis for PD-L1, total ERK, phospho ERK and β-actin were done for all treatment combination mentioned above (* indicate p<0.01, ** indicate p<0.001, ns- not significant).