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. 2016 Jul 11;6:29337. doi: 10.1038/srep29337

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Copyright © 2016, Macmillan Publishers Limited

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Alignments of Dsx isoforms among five coleopteran species (Gc, Gnatocerus cornutus; Tc, Tribolium castaneum; Td, Trypoxylus dichotomus; Ot, Onthophagus taurus; Cm, Cyclommatus metallifer). Highlighted residues in grey, light pink, dark pink, orange and light blue indicate conserved residues among five species in OD2 region, female-specific region, female-short isoforms, female-long isoforms and male isoforms, respectively. Conserved OD2 domain (Pfam08828) sequences are aligned with Dsx sequences. Colored characters of Pfam08828 sequence indicated the residues conserved among all of five species (red) or conserved in at least two species (orange). Accession numbers of amino acid sequences of coleopteran Dsx proteins used for alignments are Gc-FS(LC105648), Tc-F2(AFQ62107), Td-FS1(BAM93344), Ot-F1(AEX92939), Cm-C(BAO23810), Gc-FL(LC105649), Tc-F1(AFQ62106), Tc-F3(AFQ62108), Td-FL1(BAM93341), Ot-F2(AEX92940), Ot-F3(AEX92941),Ot-F4(AEX92942), Ot-F5(AEX92943), Cm-D(BAO23811), Gc-M(LC105647), Tc-M(XP_001807448), Td-M1(BAM93339), OT-M(AEX92938) and Cm-B(BAO23809).