Table 2. Descriptions of fourteen candidate ICGs in V. volvacea and parameters derived from RT-qPCR analysis.
| Gene symbol | Gene name | Accession number | Primer sequences (5′–3′) | Product size (bp) | PCR efficiency (100%)a | Annealing temperature (°C) |
|---|---|---|---|---|---|---|
| 18S | 18S ribosomal RNA | KF471312 | F: TCTTGTGAAACTCTGTCGTGCTGGGR: TTGCCCACACCCCAAAGCTAATTCG | 186 | 1.87 | 66 |
| ACTB | β-actin | KF528321 | F: AGCTCTCTTCCAACCTGCCTTCTTGR: GCGACAATCTTAACCTTCATGCTTGC | 216 | 1.98 | 62 |
| CYP | Cyclophilin | KF528322 | F: GATCCCAATTCTTCGTCACCACTGR: ACCACTTGCAGATCCAAAGCCCTCG | 125 | 1.97 | 63 |
| GAPDH | Glyceraldehyde 3-phosphate dehydrogenase | KF528323 | F: ATTGGCGTGGTGGTCGTAGR: ACGGAAACATCAAGGGTAGGG | 142 | 1.95 | 60 |
| HH3 | Histone H3 | KF528324 | F: GTGAAATCAGACGTTACCAGAAGTR: TGGGTTGGATAGTGACACGCTTAG | 219 | 2.03 | 59 |
| α-TUB | α-tubulin | KF528325 | F: ACTTGGTTCCTTTCCCTCGTATTCACR: GCCATGTATTTGCCTTCCCTAG | 169 | 2.02 | 61 |
| β-TUB1 | β-tubulin 1 | KF528326 | F: GATTCCCAACAACGTCCTCACCR: TCAGCTTCCGTGAACTCCATCTC | 204 | 1.90 | 61 |
| β-TUB2 | β-tubulin 2 | KF528327 | F: GAACTCAAGAGACATTCGCTGCCR: TTCACCATCCTCCACAGACGCTTC | 164 | 1.94 | 63 |
| UBQ | Ubiquitin | KF528328 | F: GGAAAGACGATCACCTTGGAGR: AGGCGAAGAACAAGATGAAGAGTG | 191 | 1.95 | 60 |
| L-asp | L-asparaginase | KF528333 | F: TTCGTGTCGCTCTCTCTCCAGR: GTATTGACTGTTTGTGAGGCTTGT | 141 | 2.00 | 59 |
| Ras | Ras protein | KF528332 | F: ACATACGAGAGAGAAGTTTCCAAGR: TCTCCTCCTTCTGTGGCTTGAC | 181 | 2.13 | 59 |
| SPRYp | Endosomal SPRY domain protein | KF528330 | F: CTCCTCCTCCAATAACCCCCATCR: TCAGAGTCTATTCCTTGCCTATCCG | 181 | 2.03 | 62 |
| Vps26 | Vacuolar protein sorting associated protein 26 | KF528331 | F: CGATTCGACTGTTCCTTGGTGGR: TGCTTGAAATATCGGCGGTTCTC | 127 | 1.99 | 61 |
| MSF1 | MSF1 domain containing protein | KF528329 | F: CAGATGGACTAGAGAAGTGGTTAGR: ATCTCTTATGAAGGTGCGTGTG | 136 | 1.94 | 59 |
a2 represents PCR amplification efficiency is 100%.