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Increased expression of cas1 and cas2 leads to primed adaptation from fully matching protospacer targets. (A) An agarose gel showing the results of separation of products of PCR amplification of CRISPR arrays from aliquots of KD263 (lanes 1 and 2) and KD546 (lanes 3 and 4) transformed with indicated plasmids. (B) Results of mapping of spacers acquired by KD263 cultures transformed with pG8mut (2) and KD546 cultures transformed by pG8 and pG8mut (3, 4) on donor plasmids.
