Fig. 1.

Bcl11b's deficiency in iNKT cells causes a major reduction in thymic and peripheral iNKT cells associated with increased apoptosis. (A and B) Frequencies (A) and absolute numbers (B) of iNKT cells from Bcl11b^F/F/PLZF-Cre (KO) and WT mice in thymus, spleen, and liver. The gated population in A shows percentages of CD1d-PBS-57⁺cells. (C) Frequencies of Annexin V⁺ iNKT cells from Bcl11b^F/F/PLZF-Cre (KO) and Bcl11b^F/F (WT) mice in thymus and spleen. Data are representative of several independent experiments with 10 pairs of mice (A and B) and 5 pairs of mice (C). P values determined by unpaired two-tailed Student’s t test are indicated. Means ± SEM.