Fig. 5.

PLZF directly binds Bach2. (A) Immunoblot of lysates from indicated cell types with anti-Bach2, anti-PLZF, and anti-GAPDH. Ratios of Bach2 over GAPDH band density are indicated. Data are representative of two independent experiments. (B, Upper) PLZF ChIP-seq reads aligned at the Bach2 gene locus. Peaks are indicated by arrows. (Lower) For ChIP-qPCR analysis, the fold enrichment is normalized relative to enrichment at the promoter region of reference gene Amhr2. Data are shown as mean ± SD of two to four independent experiments. *P < 0.05, **P < 0.01.