Cryo-EM reconstructions of EVN- and AVI-SRC. (A and B) Chemical structures of the orthosomycins AVI (A) and EVN (B), with compositional differences highlighted. (C and D) Cryo-EM electron densities (gray mesh) with fitted models for AVI (red; C) and EVN (yellow; D). (E) Overview of EVN/AVI binding site on the 70S ribosome (50S, gray, and 30S subunit omitted for clarity). Binding position of EVN/AVI (yellow) is shown relative to the P-site tRNA (blue), ribosomal protein L16 (cyan), H89 (green), and H91 (red).