Interactions of EVN and AVI with the ribosomal protein L16. (A) Overview of L16 (blue) interactions with EVN (gold) and AVI (red). (B and C) Close-up views of showing interactions between Arg-51, -55, and -59 of L16 (blue) and ring A of AVI (B) and rings A and A′ of EVN (C). (D) Sequence alignment of the L16 from E. coli (E.c), B. subtilis (B.s), E. faecalis (E.fl), E. faecium (E.fc), S. aureus (S.a), and S. pneumoniae (S.p), with residues conferring resistance to EVN and AVI highlighted in red.