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EVN and AVI block ribosomes at the start codon of the mRNA. Toe-printing assay monitoring translation in the presence of increasing concentrations (1, 10, and 100 μM) of EVN or AVI is shown. Additionally, control reactions without antibiotic (−) or including thiostrepton (Ths, 100 μM) or edeine (Ede, 50 μM) are shown. AUG designates location of the ribosomes stalled at the start codon. C, U, A, and G indicate the sequencing lanes.
