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. 2016 Jun 16;113(27):7596–7601. doi: 10.1073/pnas.1600934113

Fig. S3.

Fig. S3.

Additional phenotypes of Pip4k2c−/− mice. (A) Plasma levels of liver function parameter proteins and metabolites. Plasma of the mice were collected and analyzed for the level of the proteins and metabolites indicated (three 12-mo-old mice per genotype). The results are presented as means ± SE of the means. ALT, alanine transaminase; AST, aspartate transaminase; BUN, blood urea nitrogen. (B) Levels of rheumatoid factor in plasma. Rheumatoid factor was measured using mouse rheumatoid factor Ig ELISA kit (6200, Alpha Diagnostic International) according to the manufacturer’s protocol (three 12-mo-old mice per genotype). The results are presented as means ± SE of the means. (C) Gross observation of Pip4k2c−/− mouse livers. (Left) Wild type; (Right) Pip4k2c−/− (12 mo old). Pale color livers were often found in Pip4k2c−/− mice older than 12 mo. (D) Pip4k2b−/− Pip4k2c−/− mice are not viable at the developmental stage. Table showing the expected and observed genotype frequency (%) of 3-wk-old pups from breeding Pip4k2b+/− Pip4k2c+/− with Pip4k2b+/− Pip4k2c−/−, Pip4k2b+/− Pip4k2c+/−, or Pip4k2b−/− Pip4k2c+/− mice. Expected percent frequency is provided in parentheses. n = 64.