Skip to main content
. 2016 Jun 22;113(27):7563–7568. doi: 10.1073/pnas.1603930113

Fig. 4.

Fig. 4.

Dlx5/6 represses the H1PA/OFT enhancer within crNCCs. (A) Luc assays show that DLX5/6 represses H2/BMP synergistic activation of the H1PA/OFT enhancer. (B–E) In situ hybridization shows that crNCC H2 expression (B and C) is lost in Smad4CKOs, whereas Dlx5 expression (D and E) is uniformly expressed throughout MD1. (F–I) H1 expression is expanded proximally in caBmpr1a F0 embryos (F and G) and Dlx5 expression (H and I) is further restricted to the proximal PAs. (J) EMSAs show that DLX5 can bind to three discrete sites within the H1 enhancer. *, bound DNA complexes. Dotted lines denote where gel lanes were rearranged. Unlabeled competitors are as follows: 5′m, DLX/GATA 5′mut; 3′m, GATA 3′mut; 5′/3′ m, DLX/GATA 5′, GATA 3′mut; I2, MD1mut; mutant; WT, wild-type.