Table S1.
Construct no. | From | To | Length, bp | 5′ restriction site | 3′ restriction site |
13 | −34,680 | −32,285 | 2,395 | XhoI* | SalI* |
14 | −34,680 | −33,254 | 1,426 | XhoI* | KpnI |
15 | −33,254 | −32,285 | 969 | KpnI | SalI* |
16 | −33,254 | −32,828 | 426 | KpnI | DraI |
17 | −32,828 | −32,576 | 252 | DraI | SfoI |
18 | −32,576 | −32,285 | 291 | SfoI | SalI* |
19 | −32,828 | −32,285 | 543 | DraI | SalI* |
20 | −32,828 | −32,385 | 443 | DraI | AluI |
21 | −34,680 | −32,828 | 1,852 | XhoI* | DraI |
XhoI and SalI sites were introduced via TOPO cloning.