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. 2016 Jun 22;113(27):7563–7568. doi: 10.1073/pnas.1603930113

Table S1.

Genomic location of Hand1 enhancer constructs relative to the Hand1 transcriptional start site

Construct no. From To Length, bp 5′ restriction site 3′ restriction site
13 −34,680 −32,285 2,395 XhoI* SalI*
14 −34,680 −33,254 1,426 XhoI* KpnI
15 −33,254 −32,285 969 KpnI SalI*
16 −33,254 −32,828 426 KpnI DraI
17 −32,828 −32,576 252 DraI SfoI
18 −32,576 −32,285 291 SfoI SalI*
19 −32,828 −32,285 543 DraI SalI*
20 −32,828 −32,385 443 DraI AluI
21 −34,680 −32,828 1,852 XhoI* DraI
*

XhoI and SalI sites were introduced via TOPO cloning.