Amino acid sequence alignment of tde-linked vgrG homologs. Identified VgrG1 and VgrG2 homologs are aligned and the amino acid residues in dark gray representing highly conserved residues or in light gray indicate partially conserved residues. Numbers at the right represent the amino acid residue positions of each aligned VgrG homolog. Gene ID of VgrG proteins from top to bottom: 15158894, 745837943, 393183390, 749199676, 574594253, 652473941, 211959592, 517049910, 659658772, 647796246, 528839901, 217394189, 714469101, 705577537, 498139720, 447019410, 487841774, 262025037, 159185839.