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Inactivation of Rfwd2 in the lung epithelium results in defects in lung branching morphogenesis. Representative control (A, C, E, and G) and Shhcre;Rfwd2 mutant (B, D, F, and H) whole lungs from E11.5 to E13.5 with the epithelium outlined by anti-CDH1 (also termed E-cadherin) immunohistochemical staining. (A and B) Red lines outline the epithelium between lateral secondary branch (L) 1 and L2. (Scale bars: 500 μm.)
