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. 2016 Jun 22;113(27):7557–7562. doi: 10.1073/pnas.1603310113

Fig. 3.

Fig. 3.

Inactivation of Rfwd2 altered proximal–distal airway patterning. (A–D) Representative RNA in situ hybridization for Sox2 or Sox9 at E12.5 in Shhcre;Rfwd2 mutants and littermate controls. Arrowheads indicate the distal ends of the Sox2 domain. Sox9 expression in the trachea and extrapulmonary bronchi is in the mesenchymal precursors of the cartilage but not in the epithelial cells, as is its distal expression domain. (Scale bars: 500 μm.) (E) mRNA quantification by qRT-PCR of E12.5 lungs (Sox2: 1.00 ± 0.26 for controls, 0.37 ± 0.12 for Shhcre;Rfwd2 mutants, P = 0.004; Sox9: 1.00 ± 0.068 for controls, 1.51 ± 0.21 for Shhcre;Rfwd2 mutants, P = 0.005). Quantification was carried out in n ≥ 3 samples. **P < 0.01.