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Inactivation of Rfwd2 led to increased expression of JUN and C/EBPα proteins. (A) Western blot of JUN and C/EBPα proteins in E12.5 lungs with indicated genotypes. ACTB was used as a loading control. (B and C) Quantification of Western blot normalized to ACTB. The density of bands was measured using the Image Studio Lite program. *P < 0.05; **P < 0.01.
