Table S1.
Summary of fluorescence profile and functional activity of GluA2-YFP constructs expressed in HEK293 cells and Xenopus oocytes
| Construct | Insertion site† | Fluorescence intensity | Glutamate EC50,‡ μM |
| WT GluA2 | — | — | 73 [62–87] |
| GluA2m | — | — | 89 [78–101] |
| GluA2-0Y | ND˅IQ (26–29) | Bright | 44 [28–68] |
| GluA2-1Y | PG˅AH (286–289) | Bright | 62 [51–76] |
| GluA2-2Y | PA˅VP (334–337 | Dim | n.d. |
| GluA2-3Y | ELP˅S (401–404) | Bright | 46 [37–59] |
| GluA2-4Y | DPL˅AY (540–544) | Dim | n.d. |
| GluA2-5Y | WM˅CIV (547–551) | None | n.d. |
| GluA2-6Y | LVS˅RF (563–567) | Bright | 40 [29–57] |
| GluA2-7Y | RET˅QS (581–585) | Bright | 83 [68–101] |
| GluA2-8Y | LSG˅RI (617–621) | Dim | n.d. |
| GluA2-9Y | SYT˅AN (636–640) | Dim | n.d. |
| GluA2-10Y | SQN˅SQ (860–864) | Bright | 23 [19–28]* |
n.d., not determined.
*
P < 0.05 value statistically significantly different from WT GluA2.
†
Amino acid sequence and position of insertion sites (denoted by ˅) within rat GluA2.
‡
EC50 values were determined as described in Materials and Methods. Data represent EC50 obtained from fitting of composite dose–response data from experiments at three to six oocytes (95% confidence intervals in brackets).