Skip to main content
. 2016 Jun 17;113(27):E3872–E3881. doi: 10.1073/pnas.1605074113

Fig. 6.

Fig. 6.

Competition or synergy in binding of Fabs and fibronectin. K562 cells were incubated with high concentrations of Fabs or Fn3 9–10 (Fn) effectors for 90 min (labeled on left axis) without (A–I) or with (J–O) 15 µM Fn, and then with a second Alexa Fluor 488-labeled Fab or Fn reporter at the concentrations shown (Top) for 30 min and subjected to immunofluorescence flow cytometry without any washing. Effector concentrations are (A–I) 3 μM for TS2/16 and 12G10; 6 μM for 8E3, 9EG7, SNAKA51, SG19, and mAb13; 15 μM for HUTS-4 and Fn; and (J–O) 20 nM for TS2/16 and 12G10; 40 nM for 8E3, 9EG7, and SNAKA51; and 100 nM for HUTS-4. Error bars show standard deviation.