The Allen Brain Observatory. This in-house project is producing neuronal activity data from genetically identified neuronal populations in the behaving mouse using two-photon calcium imaging. (A) Responses are recorded to a variety of visual stimuli, including gratings, sparse noise, natural images, and natural movies, and across multiple regions, layers, and genetically defined cell types. (B) Example single-neuron response properties. Upper shows receptive fields from locally sparse noise separately for on (white) and off (black) stimuli (Fig. 3A). Lower shows example tuning curves for orientation and spatial frequency obtained from such imaging (in units of DF/F) in response to oriented gratings (cpd, cycles per degree; DF/F, relative change in fluorescence).