Fig. 1.

Regulation of protein synthesis by FGF signaling in chondrocytes. (A–C) Indicated cells were treated with FGF1, and protein synthesis activity was measured by S³⁵-Met/Cys incorporation. Relative levels of radioactivity incorporated are compared with untreated cells. Lysates were normalized to the amount of total protein. The data are representative of three independent experiments, error bars represent mean ± SD, and P value is indicated when significant. (D) C₃H₁₀T1/2 cells were differentiated into chondrocytes (C), adipocytes (A), and osteoblasts (O). After 11 d in differentiation medium, cells were treated with FGF1, and protein synthesis activity was measured as described above.