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. 2016 Jun 17;113(27):7551–7556. doi: 10.1073/pnas.1600363113

Fig. 5.

Fig. 5.

Col-I–expressing cells form perivascular structures. (A) COL-EGFP+ cells are found in close association with tumor vessels. COL-EGFP+ structures wrap the DsRed+ vessels in MC57 fibrosarcoma and MC38 carcinoma growing in DsRed+COL-EGFP+ chimeric mice with an EYFP+ BM. Representative images are shown from two out of three independent longitudinal experiments performed with MC57, MC38, and Pro4L-Cerulean tumors (n = 1 mouse each). COL-EGFP+ cells wrapping vessels were observed in normal (non-BM chimeric) COL-EGFP mice in five more independent experiments. (B) SMA-DsRed+ cells do not form tubular structures around vessels in late-stage established tumors. In day 22–25 MC57 and MC38 tumors grown in COL-EGFP/SMA-DsRed dual reporter mice, structures that are COL-EGFP+ but SMA-DsRed− wrap the vessels. DiD-labeled red blood cells (DiD-RBC) were injected i.v. to visualize blood flow. Representative images are shown from two out of three independent longitudinal experiments performed using Cerulean-expressing MC57, Pro4L, and MC38 (n = 1 mouse each). The host was Rag−/− for the MC57 and Pro4L tumors.