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. 2016 Jun 13;113(27):E3901–E3910. doi: 10.1073/pnas.1523512113

Fig. 8.

Fig. 8.

Inhibition of OX40L prevents the development of fibrosing alveolitis: histological analysis. (A) Representative H&E-stained lung sections from Fra-2 mice treated with control IgG (n = 7), Fra-2 mice treated with anti-OX40L mAb (n = 8), and control C57/BL6 mice (n = 10). (Scale bar: 100 µm.) (B) Lung fibrosing score was significantly higher in Fra-2 mice receiving control mAb than in Fra-2 mice treated with anti-OX40L mAb. (C) The hydroxyproline assay evaluates collagen content. (D) SHG showed fibrillar collagen (in pink) in Fra-2 mice treated with control IgG. (Scale bar: 50 µm.) (E) Second harmonic scores were higher in Fra-2 mice receiving control IgG than in Fra-2 mice treated by anti-OX40L mAb or in control mice. Twenty-five mice were used for these experiments. Values in B, C, and E are represented by dot blots with means ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; two-sided Mann–Whitney test. AU: arbitrary units.